检索范围:
排序: 展示方式:
hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析
徐青,付子毅,吴小莉,皇甫玉爽,凌静
《中国工程科学》 2014年 第16卷 第5期 页码 99-104
应用实时定量聚合酶链式反应(PCR)技术检测子宫肌瘤组织中的hsa-miR-197 表达水平,并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197 序列保守性及其基因组特征。选择miRanda、MirTarget2 及TargetScan 三个在线数据库预测hsa-miR-197 的靶基因并取交集以便后续分析。通过基因本体(GO)功能注释、GO富集分析和信号转导通路富集分析初步阐明,hsamiR-197 靶基因参与调控的细胞功能与信号通路。hsa-miR-197 的功能较广泛,参与肿瘤发生的多种生物学过程,提示hsa-miR-197 可能与子宫肌瘤的发病机制密切相关。
关键词: 子宫肌瘤 hsa-miR-197 生物信息学 靶基因
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 97-109 doi: 10.1007/s11684-016-0496-1
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.
关键词: condylar cartilage mechanical loading proteomic analysis iTRAQ bioinformatics analysis
Bioinformatic exploration of MTA1-regulated gene networks in colon cancer
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 178-182 doi: 10.1007/s11684-016-0442-2
Metastasis-associated gene 1 (MTA1) controls a series of biological processes in tumor progression. Tumor progression is a complex process regulated by a gene network. The global cancer gene regulatory network must be analyzed to determine the position of MTA1 in the molecular network and its cooperative genes by further exploring the biological functions of this gene. We used TCGA data sets and GeneCards database to screen MTA1-related genes. GO and KEGG pathway analyses were conducted with DAVID and gene network analysis via STRING and Cytoscape. Results showed that in the development of colon cancer, MTA1 is linked to certain signal pathways, such as Wnt/Notch/nucleotide excision repair pathways. The findings also suggested that MTA1 demonstrates the closest relationship in a coregulation process with the key molecules AKT1, EP300, CREBBP, SMARCA4, RHOA, and CAD. These results lead MTA1 exploration to an in-depth investigation in different directions, such as Wnt, Notch, and DNA repair.
关键词: metastasis-associated gene 1 colon cancer bioinformatics
15th International Congress for Stereology and Image Analysis 第十五届国际体视学与图像分析学术会议
会议日期: 2019年05月27日
会议地点: 丹麦/奥胡斯
主办单位: 国际体视学与图像分析学会(ISSIA)
福田敏男:Bio Cell Analysis and Assembly by Micro and Nano Robotic Technology(2019年8月25日)
福田敏男(院士)
2021年01月20日
关键词: 采油工程
Decomposition analysis applied to energy and emissions: A literature review
《工程管理前沿(英文)》 页码 625-639 doi: 10.1007/s42524-023-0270-4
关键词: index decomposition analysis structural decomposition analysis production decomposition analysis energy CO2 emissions
B. VIDHYA,K. N. SRINIVAS
《能源前沿(英文)》 2016年 第10卷 第4期 页码 424-440 doi: 10.1007/s11708-016-0423-9
关键词: flux reversal generator air velocity computation fluid dynamics thermal analysis vibration analysis finite element analysis
Review of sentiment analysis: An emotional product development view
《工程管理前沿(英文)》 页码 592-609 doi: 10.1007/s42524-022-0227-z
关键词: sentiment analysis emotion product development Kansei Engineering
Jack up reliability analysis: An overview
Ahmad IDRIS, Indra Sati Hamonangan HARAHAP, Montasir Osman Ahmed ALI
《结构与土木工程前沿(英文)》 2018年 第12卷 第4期 页码 504-514 doi: 10.1007/s11709-017-0443-5
关键词: jack up reliability analysis uncertainty analysis review of jack up
Systems biomedical analysis of Schistosoma japonicum
Ze-Guang HAN MD,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 157-165 doi: 10.1007/s11684-010-0034-5
Pengxing YI,Peng HUANG,Tielin SHI
《机械工程前沿(英文)》 2016年 第11卷 第4期 页码 388-402 doi: 10.1007/s11465-016-0404-z
Wind turbine gearbox (WTG), which functions as an accelerator, ensures the performance and service life of wind turbine systems. This paper examines the distinctive modal properties of WTGs through finite element (FE) and experimental modal analyses. The study is performed in two parts. First, a whole system model is developed to investigate the first 10 modal frequencies and mode shapes of WTG using flexible multi-body modeling techniques. Given the complex structure and operating conditions of WTG, this study applies spring elements to the model and quantifies how the bearings and gear pair interactions affect the dynamic characteristics of WTGs. Second, the FE modal results are validated through experimental modal analyses of a 1.5 WM WTG using the frequency response function method of single point excitation and multi-point response. The natural frequencies from the FE and experimental modal analyses show favorable agreement and reveal that the characteristic frequency of the studied gearbox avoids its eigen-frequency very well.
关键词: wind turbine gearbox modal analysis finite element analysis modal frequency bearing equivalence
Isogeometric analysis of coupled thermo-elastodynamic problems under cyclic thermal shock
Asghar AMANI DASHLEJEH
《结构与土木工程前沿(英文)》 2019年 第13卷 第2期 页码 397-405 doi: 10.1007/s11709-018-0473-7
关键词: isogeometric analysis coupled thermo-elastodynamic dynamic analysis generalized newmark cyclic thermal shock
A dimensional analysis on asphalt binder fracture and fatigue cracking
Qian ZHAO, Zhoujing YE
《结构与土木工程前沿(英文)》 2018年 第12卷 第2期 页码 201-206 doi: 10.1007/s11709-017-0402-1
A frequency error estimation for isogeometric analysis of Kirchhoff–Love cylindrical shells
《结构与土木工程前沿(英文)》 doi: 10.1007/s11709-023-0006-x
关键词: isogeometric analysis Kirchhoff–Love cylindrical shell free vibration frequency error convergence
标题 作者 时间 类型 操作
iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar
null
期刊论文
15th International Congress for Stereology and Image Analysis 第十五届国际体视学与图像分析学术会议
2019年05月27日
会议信息
黄幼麟:Dynamic Consumer Preferences for Electric Vehicles — A Longitudinal Analysis(2020年7月12日)
2022年06月10日
会议视频
福田敏男:Bio Cell Analysis and Assembly by Micro and Nano Robotic Technology(2019年8月25日)
福田敏男(院士)
2021年01月20日
会议视频
Flow, thermal, and vibration analysis using three dimensional finite element analysis for a flux reversal
B. VIDHYA,K. N. SRINIVAS
期刊论文
Jack up reliability analysis: An overview
Ahmad IDRIS, Indra Sati Hamonangan HARAHAP, Montasir Osman Ahmed ALI
期刊论文
Numerical analysis and experimental investigation of modal properties for the gearbox in wind turbine
Pengxing YI,Peng HUANG,Tielin SHI
期刊论文
Isogeometric analysis of coupled thermo-elastodynamic problems under cyclic thermal shock
Asghar AMANI DASHLEJEH
期刊论文